Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
MTC Bio qPCR Sealing Film, Optically Clear, Non-Tacky Pressure Activated Adhesive, Sterile, 10 bags of 10 seals, 100/pk
PureAmp™ Sealing Films are compatible with all standard PCR plates.Film and foil options include standard adhesive pierceable aluminum, standard adhesive transparent, optically clear standard adhesive, optically clear non-tacky pressure activated adhesive, and low-adhesive EZ-peel, all of which are guaranteed to keep evaporation rates below 5%.• Multiple types for PCR, qPCR, and ELISA• All types are available sterile• Evaporation rates < 5%• 10 inner bags of 10, 100 per pack• Certified RNase, DNase, DNA, & PCR inhibitor free
Discovery Life Sciences Ovarian Cancer DTC
DTC Dissociated Tumor and Tissue Cells start as fresh solid tumor tissue collected during surgery are enzymatically and mechanically dissociated into single cells and are delivered as viably cryopreserved cells for functional assays cell culture and single cell genomics
Alkali Scientific IsoFreeze Color Changing qPCR Racks, 2 Racks/Unit, Blue to White
•Maintain the thermo sensitive samples temperature stable at 4 ° C for 4 hours • Change color from Light Blue to White as it warms • Supplied with lid • The contained gel is nontoxic and non hazardous PCR Racks: • Dim. (mm) 44 x 99 x 141 with lid / 38 x 99 x 141 without lid • PCR Version for 96 plates or strips or PCR 0.2 ml microtubes(8 x 12 emplacements) • qPCR Version for 64 Corbett Rotor Genetubes, or 16 tubes 0.2 ml and 4 tubes 0.5 ml Racks for tubes 0.5, 1.5 or 2 ml: • Dim. (mm): 70 x 105 x 146 with lid / 64 x 105 x 146 without lid • For 24 tubes (6 x 4 emplacements)
Andwin Scientific QSCRIPT 1STEP TOUGHMX 500X20UL
The qScript XLT One-Step RT-qPCR ToughMix by Quanta Biosciences is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates with the use of hybridization probe detection chemistries like the TaqMan 5-hydrolysis probes on real-time PCR systems that do not need a passive reference dye. This product contains: reagent description, qScript XLT One-Step RT-qPCR ToughMix (2X), 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, AccuVue blue qPCR dye, and stabilizers.
Eagle Biosciences Inc E.coli Residual DNA Detection Kit (qPCR)
E.coli Residual DNA Detection Kit (qPCR)
Bioss SARSCovid Coronavirus NP
SARS Covid Coronavirus NP Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications WB ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Meridian Life Science Lyo-Ready qPCR Mix, 100ML
Lyo-Ready qPCR Mix, 2x is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2 and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.
Perkin Elmer US LLC 5-Position Priority Samples Rack
This rack features inserts for 8 mL tube (N8145125), 15 mL tube (N0777471), 50 mL tube (N8145124), 125 mL bottle (N0777820) and 250 mL bottle (N0777818). For PerkinElmer prepFAST only.
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
INTACT GENOMICS INC ig SYBR Green qPCR 2X Master Mix 500 Reactions
Intact Genomics SYBR Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA by qPCR. The Ig SYBR Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. This features a high-quality hot start Taq DNA Polymerase for higher fidelity and better amplification.Applications Gene expression validation Absolute quantification Mutation detection Pathogen detection GMO characterization Genetic profilingBenefits Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55 °C.
INTACT GENOMICS INC igScript™ Probe-Based qPCR master mix 2500 reactions
IgScript™ Probe-Based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye in a standard buffer. It provides improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase Enhanced efficiency, specificity, and sensitivity. Compatible with all real-time PCR instruments for Gene expression validation, multiplexing, mutation detection, pathogen detection and GMO characterization.
NEW ENGLAND BIOGROUP 10 boxes of 50 LUNA-FX7 or LUNA-BX7 8-channel ultra low fluorescent counting slides, 500 slides/4000 counts. For LUNA-FX7 and LUNA-BX7.
10 boxes of 50 LUNA-FX7 or LUNA-BX7 8-channel ultra low fluorescent counting slides, 500 slides/4000 counts. For LUNA-FX7 and LUNA-BX7.
LUNA NANOTECH INC Ngsprep Size Selection Magnetic Beads, 50 ML
PuroMAG™ NGSPrep Magnetic Beads, 50 mL
The PuroMAG™ NGSPrep Magnetic Beads are designed for fast and highly effective DNA clean-up after polymerase chain reaction (PCR, qPCR, ddPCR), microarrays, sequencing and other types of ezymatic reactions by removing unincorporated primers, dNTPs (deoxynucleotide triphosphates), salts, and other impurities. Combination of SPRI technology and paramagnetic feature of the beads allows for specific size selection of DNA fragments during the clean-up procedure with high recovery of the DNA product. This is crucial application in library preparation for various sequencing techniques, including next-generation sequencing (NGS).
Features
- Easy and fast clean-up of DNA PCR samples with efficient recovery of the product
- Highly efficient size selection of purified fragmented genome or PCR product for NGS library prep
- High throughput clean-up of 96/384 samples
- Can be used o
LUNA NANOTECH INC Maxi Silica Spin Columns, 50 Columns
PuroSPIN™ MAXI Spin Columns for DNA / RNA Extraction and Purification, 50 columns
Have you ever ran out of columns that come with your favorite plasmid extraction kit but still had a lot of left-over buffer reagents remaining? Instead of buying a new expensive kit you can use PuroSPIN™ columns with the left-over buffers as a cost-effective alternative. Simply use PuroSPIN™ columns while following the manufacturer-specific protocols.
Nucleic acid binding capacity: 2.3 mg of genomic DNA, 500 µg of plasmid DNA
Compatible as cost-effective replacement columns for Plasmid DNA purification kits from Qiagen, ThermoFisher, Bio-Rad, Promega and other manufacturers
PuroSPIN™ MAXI silica spin columns are designed for fast, simple and efficient extraction of Plasmid DNA from bacterial cultures
Detroit R & D DNA DAMAGE ANALYSIS KIT
Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit